Fig. S8.

Expression profiles of melanin synthesis-related genes in the larval epidermis. Temporal expression patterns of genes related to melanin synthesis in larval epidermis from the third to fourth ecdysis (n = 3 in each period and strain) are shown in the mutant Ze (A, BmTH; C, BmDDC; E, Bmlac-2; G, Bmyellow) and the WT strain (B, BmTH; D, BmDDC; F, Bmlac-2; H, Bmyellow). Expression of the BmrpL3 gene was used as an internal control. Head capsule slippage and pigmentation period are denoted by HCS (orange triangle and broken line) and P (gray box), respectively, and ecdysis is denoted by E (black triangle). Black circles and white diamonds indicate the pigmented and nonpigmented areas of the Ze mutant (or corresponding area in the WT strain), respectively, which are shown as red and blue boxes in the abdominal third segment, respectively. Error bars indicate the SD.