Table S2.
Primers for cloning, 5′ and 3′ RACE of candidate genes, construction of overexpression plasmids, and qRT-PCR
| Target gene and name | Sequence (5′ → 3′) |
| Cloning | |
| Bm8846 | |
| 8846-F3 | ATGCTACGAACGCCACTACGC |
| 8846-F5 | CCGGGTCAAACTTTGGACTT |
| 8846-F6 | GCTGGACACAAGGATTCGTC |
| 8846-R3 | TTATGTATCTTTGTTTGCATCCTTGGC |
| 8846-R4 | GCTTCCAATCAACATCATCG |
| 8846-R5 | ACATTTATGTATCTTTGTTTGCATCC |
| 8846-R6 | TGGACTGCAGTGTGCAGCTCCA |
| Bmspz-3 | |
| 9035-F3 | TGGTGTTAGCACGAGCGGACAG |
| 9035-F4 | CGATGAGAAACTCAGTGGGCTGTG |
| 9035-F5 | AGAGTGTATGTGGCATCGGTGCGA |
| 9035-F6 | GCATGTCATGTCTTGCCGCCATAAC |
| 9035-R9 | GTCGAAGCCGGACTGAAGCCTT |
| 9035–5RACE-F2 | AGTCAGTAGTGGTCGCGCGCGA |
| 9036-F4 | ATAGAACACTGCCATTGTATTGTTACG |
| 9036-R10 | CCGTCAAGGGAATGAAAATTACAAATC |
| 9036-R3 | CCGGTCGTGGTTTTGCTGCAAGTT |
| 9036-R4 | GCTTTGATTTGCGGAGAGTGAAGTGA |
| 9036-R5 | AGTTTCCAGCCGTTGGGCAATACAA |
| Bmago-3 | |
| 9037-F3 | ATGATGGACTCTCCCTCACAATCT |
| 9037-F4 | CGCACACGTCGTTTGAGCGAA |
| 9037-F5 | AGATTTCATGATATGCCTCATTCCGG |
| 9037-R3 | CTGTTACGTATCCAGGCCAAACTT |
| 9037-R4 | TAGACGACTCTTGGCAGTTTCGTT |
| 9037-R5 | GTATGCTGATGCTCCAAAGCGT |
| 9037-R6 | GCACGCACTGTCCCACTAGAT |
| 5′ and 3′ RACE | |
| Bm8846 | |
| 8846–3RACE-F1 | GCCAGCGGACTGCCTGTGGTAACAGATGC |
| 8846–3RACE-nest-F1 | ATCGTGCTCTTCTTGTCCAAGCT |
| 8846–5RACE-nest-R1 | GCGTAGTGGCGTTCGTAGCAT |
| 8846–5RACE-R1 | GGCAAGTGGACTGCAGTGTGCAGCTCCA |
| Bmspz-3 | |
| 9035–5RACE-nest-R1 | ACTTTCCGCTCGTGACGTCC |
| 9035–5RACE-R1 | GAGTGGCGTGGGATCGTCTACACCGAACGGG |
| 9036–3RACE-F1 | TTCGACGCCGCCCGGCAGCCGAGTGAAG |
| 9036–3RACE-F2 | GCCTGACGCGCAGGCCGTCAACAGTACCG |
| 9036–3RACE-F3 | CTCGTTTACAGACGTGACCACA |
| 9036–3RACE-nest-F1 | GGTGGATGCGTGCGAAAGCAA |
| 9036–3RACE-nest-F2 | CCCTCAACTCCGCAAGGAAACT |
| 9036–3RACE-nest-F3 | ATCAGTACCAAAACTTGTTGGAGGC |
| Bmago-3 | |
| 9037–3RACE-F3 | CCGAATCCCGGGACCGTGGTCGATCACTGT |
| 9037–3RACE-F4 | ACACTAGGATCTTCCTGAAGTCGA |
| 9037–3RACE-nest-F3 | AACGAGACGGGACTGGTACGAT |
| 9037–5RACE-nest-R3 | CCTATACTCACTCCTGAAGTACTA |
| 9037–5RACE-nest-R4 | TACTAGGAGTTGCACTTGCAATGGT |
| 9037–5RACE-R3 | GCCAGCATCAATGCTGCTGCGCGTCCTCT |
| Plasmid construction | |
| Spz3ZeFL, Spz3WTFL | |
| infu-spz3-F1 | CCACCGGTCGCCACCatggctttggtaaattttacgctcgc |
| infu-spz3-R2 | AGAGTCGCGGCCGCTTCAAGGCTTACACCTGCAAACGCA |
| Spz3ZeCKfib, Spz3WTCKfib | |
| infu2-spz3-F | actaattcaaggatccaccggtcgccaccATGGCTTTGGTAAATTTTACGCTCGC |
| FibHSigP-R1 | tgcatttgtataagcgacatactgcagagcgcagcacaagat |
| Spz_CysKnot_F | GCTTATACAAATGCAcaggcctckaacacacaaytg |
| spz3CK-NotI-R1 | AGAGTCGCGGCCGCT |
| Spz3Ze(1–60), Spz3WT(1–60) | |
| BamHI-spz3(1-60)-F1 | ACTAATTCAAGGATCatggctttggtaaattttac |
| Bm_spz3_60aa_R(4mRFP) | GTCCTCGGTGTTGTCttgattcgaagggggactgaa |
| opt-mRFP-F | gacaacaccgaggacgtcatcaagga |
| 15bp-opt-mRFP-R | TCTAGAGTCGCGGCCGCctactgggagccggagtggcggg |
| qRT-PCR | |
| BmrpL3 | |
| rPL3_qPCR_03-F | ATCAAGGGTTGCTGCATGGGACCT |
| rPL3_qPCR_03-R | GGTTGATCTTTTCTAGTGCAGCCCTC |
| Bm8846 | |
| 8846_qPCR02-F | TGGTAACAGATGCTAAACCTTCGGGT |
| 8846_qPCR02-R | TGTACAGTAGGACGAATCCTTGTGTC |
| Bmspz-3 | |
| spz3qPCR01-F | CAAAACACAGCTCCACGCGGTAC |
| spz3qPCR01-R | CCGTTTCGTAGAAGTATCGGACCTTG |
| Bmago-3 | |
| 9037_qPCR02-F | GGGTTCTGTTGTTCACTCAGAGGGA |
| 9037_qPCR02-R | GAGGGGTACCAATTCAGCGTCAGA |
| BmToll-8 | |
| BmToll8_qPCR01-F | CAAGCATTGTCGGTTCTTTCTG |
| BmToll8_qPCR01-R | TTTCGGAGTGCTTGTGGATTT |
| BmTH | |
| BmTH_qPCR02-F | CGCCTTTCCACACACCTGAACC |
| BmTH_qPCR02-R | GGGATGCAAGGCCAATTTCTTGCG |
| BmDDC | |
| BmDDC_qPCR02-F | GATGAAGACATCCGCAACGGTCTCA |
| BmDDC_qPCR02-R | ATCTCCAATTTCGTCAAGAGCGTCG |
| Bmlac-2 | |
| lac-2_qPCR01-F | CGAACCACCAAGGATCTGTTACTATC |
| lac-2_qPCR01-R | GGCAATGAGACCAGACCACATTCGT |
| Bmyellow | |
| Bmyellow_qPCR01-F | GTCAGCGTTCCTAGGTGGCG |
| Bmyellow_qPCR01-R | TCGAAGCTCGGGTAGGGCGTT |