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. 2017 Jul 19;114(31):8354–8359. doi: 10.1073/pnas.1708211114

Fig. S3.

Fig. S3.

BRIT1 is dispensable for immune response in vivo. (A) Schematic of NP-CGG immunization protocol. GC, germinal center. (B) IgM, IgG1, IgG3, and IgA concentrations in serum, determined by ELISA on day 0. Ctrl, control. (C) Flow cytometric analysis of GC B cells (B220+ DAPI− GL7+ Fas+) in control, BRIT1 KO, and AID KO mice following NP-CGG immunization on day 14 and quantification of GC B cells in immunized mice on day 14 (n = 3). (D) Quantification of IgG1+ GC B cells in immunized mice on day 14 (n = 3). (E) Quantification of NP+ GC B cells in immunized mice on day 14 (n = 3). (F) Quantification of IgG1+ NP+ GC B cells in immunized mice on day 14 (n = 3). (G) IgM and IgG1 concentrations in serum from Ctrl, BRIT1 KO, and AID KO mice following NP-CGG immunization on day 28, determined by ELISA. (H) Detection of low-affinity and high-affinity NP-specific IgM and IgG1 serum antibodies in Ctrl (n = 4), BRIT1 KO (n = 4), and AID KO (n = 4) mice following NP-CGG immunization on day 28 by ELISA. Conc., concentration.