Table 1. Primers used for conventional assays and transcript preparation.
| Target | Primer | Sequence (5΄- 3΄) | Amplicon size (bp) | Reference |
|---|---|---|---|---|
| ASPV | qASP-F | TGCCTTTTACGCAAAGCATGT | 385 | This study |
| ASPV-R | TTGGGATCAACTTTACTAAAAAGCATAA | Menzel et al. [24] | ||
| ASGV | ASGV-F | CCCGCTGTTGGATTTGATACACCTC | 491 | James [38] |
| ASGV-R | CACGACTCCTA ACCCTCCAGTTCC | Massart et al. [23] | ||
| ApMV | ApMV-F | GACTTTGCCGATGTCTTCCG | 325 | This study |
| ApMV-R | GTGGTAACTCACTCGTTATCACGTAC |
Sequences of forward (F) and reverse (R) primers used for the detection of Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV) and Apple mosaic virus (ApMV) by conventional RT-PCR and the preparation of RNA transcripts.