Table 2. Primers and probes of the RT-qPCR assay.
| Target | Primer/probe | Sequence (5΄-3΄) | Reporter dye | Amplicon size (bp) | Reference |
|---|---|---|---|---|---|
| ASPV | qASP-F | TGCCTTTTACGCAAAGCATGT | NED™ | 69 | This study |
| qASP-R | GTTTGCAGGGGGACTTTGAGT | ||||
| ASP-P | TGGAACCTCATGCTGC | ||||
| ASGV | qASG-F | AGAGGACTTGCCACCAACATTT | FAM™ | 72 | |
| qASG-R | CACCCAAGGGCTTTTTTCAA | ||||
| ASG-P | AGAAATGGCCCAAAGC | ||||
| ApMV | qApM-F | TGGTGGAGGATTACGATGAAAGTA | VIC® | 66 | |
| qApM-R | TTTGAAACCCTTTCGGTCCAT | ||||
| ApM-P | CGAAAGGTCCGAATC |
Description of forward (F) and reverse (R) primers, and TaqMan MGB (P) probes used for specific detection of Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV) and Apple mosaic virus (ApMV) in the singleplex and multiplex RT-qPCR assays