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. 2017 Jul 27;12(7):e0180877. doi: 10.1371/journal.pone.0180877

Table 4. Evaluation of the multiplex RT-qPCR.

s/n Sample Host Geographic region Symptoms Multiplex RT-qPCR Conventional RT-PCR
ASPV ASGV ApMV ASPV ASGV ApMV
1 6006 Pear Peloponnese Fire blight symptomatology 34.99 35.02 - - - -
2 G02 P.amygdaliformis Central Greece Symptomless - - - - - -
3 3492 Apple Peloponnese Graft necrosis 20.03 21.74 25.58 + + +
4 B65 Pear Peloponnese Reduced leaf size, short internodes 23.93 - 36.66 + - +
5 3096 Pear Crete Absence of vegetative development - - - + - -
6 5847 Pear Thessaly Reduced leaf size, chlorosis, stunting 33.96 35.35 - + - -
7 KY1 Quince Central Greece Symptomless - - - - - -
8 2814 Apple Macedonia Chlorosis, leaf curl 22.87 23.93 27.64 + + +
9 MT Apple Peloponnese Symptomless 21.52 23.93 26.51 + + +
10 Tragic Pear Central Greece Stunting 33.7 - - + - -
11 M4 Apple Central Greece Symptomless 23.41 23.63 27.17 + + +
12 EVA2 Apple Central Greece Symptomless 21.84 - - + - -
13 2970 Pear Thessaly Necrosis of branches 33.14 35.44 - + - -
14 ELEA2 Apple Central Greece Symptomless 23.05 - 36.04 + - +
15 WIL Pear Central Greece Symptomless - - - - - -
16 4176 Pear Central Greece Symptomless 29.83 - - + - -
17 EP913 Pear Epirus Wilting 32.35 32.65 29.38 + - +
18 B39 Apple Thessaly Cankered branches 20.56 23.71 27.34 + + +
19 4215 Pear Macedonia Necrotic spots, stunting 30.85 33.55 - + - -
20 EVP2 Pear Central Greece Symptomless - - - + - -
21 3248 Pear Central Greece Leaf spots 36.63 - - - - -
22 B47 Pear Macedonia Cankered limbs, shoot necrosis 36.06 - - - - -
23 PEL5 Apple Thessaly Reduced fruit size 24.65 24.04 20.88 + + +
24 KPY Pear Central Greece Symptomless - - - - - -
25 3363 Pear Thrace Reduced leaf and branch size 35.82 36.53 - - - -
26 4608 Pear Thessaly Reduced fruit size - - - - - -
27 APL5 Apple Macedonia Symptomless 34.67 36.02 35.81 - - +
28 15643 Pear Macedonia Limbs with cankers - - - - - -
29 B70 Apple Macedonia Mosaic 25.89 24.05 24.33 + + +
30 M3 Apple Epirus Symptomless - - - - - -
31 M1 Apple Central Greece Symptomless 24.33 25.87 - + + -
32 M2 Apple Central Greece Symptomless 22.15 24.99 - + + -
33 A1 Pear Central Greece Symptomless - - - - - -
34 A2 Pear Central Greece Symptomless 26.63 25.95 - + + -
35 A3 Pear Central Greece Symptomless - - - - - -
36 A4 Pear Central Greece Symptomless 25.3 26.28 - + + -
Totala 25 18 11 22 11 11

Total RNAs isolated from thirty-six samples of pome trees derived from commercial orchards, were analysed by the developed multiplex RT-qPCR and conventional RT-PCR assays. Comparison between methods was performed on the same RNA. Samples detected positive are indicated by cycle thresholds (Ct) and (+), for multiplex RT-qPCR and conventional RT-PCR, respectively.

a Number of samples in which at least one virus was detected