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. 2017 Jul 25;2017:4218468. doi: 10.1155/2017/4218468

Figure 4.

Figure 4

Inhibition of STAT3 phosphorylation by EMP in M2 macrophages. (a) BMM were pretreated with IL-4 (20 ng/mL) + IL-13 (20 ng/mL) for 6 h to polarize to M2 and then EMP was added for 1 h. The phosphorylation of STAT3 and STAT6 was analyzed by immunoblot analysis. ND = not detected. (b) RAW264.7 cells were pretreated with IL-4 (20 ng/mL) + IL-13 (20 ng/mL) for 6 h to polarize to M2 and then EMP was added for 1 h. p-IκBα and β-actin were analyzed in the cytosolic fraction and P65 and PCNA were analyzed in the nuclear fraction. C = cytosol; N = nucleus. Values are indicated as the mean ± SEM. ∗p < 0.05 compared with the CON samples.