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The epidermal growth factor receptor (EGFR) partially mediated the hBD-3 gene induction in keratinocytes stimulated with PRGF. To analyze the influence of the EGFR on the PRGF-mediated hBD-3 induction, human primary keratinocytes were treated with PRGF (1 : 10 diluted in cell culture medium) in the presence or absence of the EGFR-blocking antibody cetuximab. HBD-3 gene expression was analyzed by real-time PCR (∗∗∗p < 0.001, ns = not significant, Student's t-test).
