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. Author manuscript; available in PMC: 2017 Aug 8.
Published in final edited form as: Science. 2011 Aug 26;333(6046):1151–1154. doi: 10.1126/science.1207203

Fig. 1.

Fig. 1

Design of tRNASep and its aminoacylation by M. maripaludis SepRS. (A) Pathway of Cys-tRNACys formation in M. maripaludis. ATP, adenosine 5′-triphosphate; AMP, adenosine 5′-monophosphate; PPi, inorganic pyrophosphate; PLP, pyridoxal phosphate. (B) Cloverleaf structure of tRNASep. Arrows indicate the three nucleotide changes compared to M. jannaschii tRNACys. (C) Acylation with Sep of M. jannaschii tRNACys and tRNASep catalyzed by M. maripaludis SepRS. Total E. coli tRNA (▼), or tRNA from E. coli strains expressing the M. jannaschii tRNACys (●) or the tRNASep (○) gene was acylated by M. maripaludis SepRS with [14C]Sep (0.1 mM) in the presence of ATP (10 mM).