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In vivo synthesis of chloramphenicol acetyl-transferase (measured by IC50 value) by tRNASep-dependent read-through of an amber mutation in the CAT gene. tRNASep was co-expressed in the E. coli Top10ΔserB strain with the proteins indicated in the figure (SepRS, M. maripaludis CysRS, SepCysS, EF-Sep, EF-Tu). Selection was carried out on LB agar plates containing 2 mM Sep and various concentrations of chloramphenicol. Error bars indicate SEM.
