Table 2.
DEGs detected by microarray chosen as good candidates for validation by RT-qPCR.
| Comparison | Gene title | Gene symbol | Microarray FC (FDR p value) | RT-qPCR p value (validation) |
|---|---|---|---|---|
| G1 + G2 versus G3 | ||||
| @213831_at #Hs03007426 mH | Major histocompatibility complex, class II, DQ alpha 1 | HLA-DQA1 | 127.80 (0.002) | <0.0001 |
| @212777_at #Hs00893134_m1 | Son of sevenless homolog 1 (drosophila) | SOS1 | 2.60 (0.005) | 0.005 |
| @222381_at #Hs00918237_m1 | Aryl-hidrocarbon receptor repressor | PDCD6 | −2.43 (0.007) | NV |
| @208368_s_at #Hs00609073_m1 | Breast cancer 2, early onset | BRCA2 | −1.46 (0.009) | 0.008 |
| @210808_s_at #Hs01071081_m1 | NADPH oxidase 1 | NOX1 | −1.43 (0.002) | NV |
|
| ||||
| G2 versus G1 | ||||
| @231857_s_at #Hs01005454_g1 | ATP/GTP-binding protein-like 5 | AGBL5 | 6.08 (0.002) | 0.042 |
| @1562028_at #Hs00236949_m1 | Cyclin D3 | CCDN3 | 1.99 (0.006) | 0.0003 |
| @213006_at #Hs00270931_s1 | CCAAT/enhancer-binding protein (C/EBP), delta | CEBPD | 1.89 (0.007) | <0.0001 |
| @214792_x_at #Hs00360269_m1 | Vesicle-associated membrane protein 2 | VAMP2 | 1.67 (0.001) | 0.0001 |
| @201313_at #Hs01102367_g1 | Enolase 2 (gamma, neuronal) | ENO2 | 1.66 (0.0008) | <0.0001 |
| @211370_s_at #Hs00177134_m1 | Mitogen-activated protein kinase 5 | MAP2K5 | 1.39 (0.001) | 0.027 |
| @212226_s_at #Hs00170359_m1 | Phosphatidic acid phosphatase type 2B | PPAP2B | −2.68 (0.005) | 0.021 |
| @223828_s_at #Hs00263821_m1 | Lectin, galactoside-binding, soluble 12 | LGALS12 | −2.09 (0.007) | 0.0004 |
|
| ||||
| G2 versus G3 | ||||
| @209480_at #Hs03054971_m1 | Major histocompatibility complex, class II, DQ beta 1 | HLA-DQB1 | 131.31 (0.005) | <0.0001 |
| @211571_s_at #Hs00171642_m1 | Versican | VCAN | 1.73 (0.009) | NV |
| @212142_at #Hs00907398_m1 | Minichromosome maintenance | MCM4 | −4.46 (0.0001) | 0.0006 |
| @239617_at #Hs01115007_m1 | SEC13 homolog (S. cerevisiae) | SEC13 | −2.34 (0.007) | 0.008 |
| @205672_at #Hs00166045_m1 | Xeroderma pigmentosum, complementation group A | XPA | −1.29 (0.003) | NV |
|
| ||||
| G1 versus G3 | ||||
| @204517_at #Hs00917412_m1 | Peptidylprolyl isomerase C (cyclosphilin C) | PPIC | 2.21 (0.008) | 0.001 |
| @1554586_a_at #Hs01598095_g1 | Rho-related BTB domain containing 2 | RHOBTB2 | 2.13(0.007) | NV |
| @1559921_at #Hs00169777_m1 | Platelet/endothelial cell adhesion molecule (CD31 antigen) | PECAM1 | 1.86 (0.004) | NV |
| @215723_s_at #Hs00160118_m1 | Phospholipase D1, phosphatidylcholine-specific | PLD1 | −3.77 (0.005) | NV |
FC = fold change; FDR = false discovery rate method according the Benjamini-Hochberg procedure was used for correcting each p value for multiple hypothesis testing; NV = microarray not validated by RT-qPCR; @ = probe set ID; # = TaqMan code of Applied Biosystems.