| Reagents (add in this order) | Volume (μl) | Final Concentration |
|---|---|---|
| End-repaired DNA w/ beads | 21.5 | |
| 10X NEBuffer 2 | 3 | 1X |
| 10 mM dATP | 3 | 1 mM |
| Klenow (exo-) (5 U/ μl) | 2.5 | 0.42U / μL |
| Total Volume | 30 |
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