| Reagents (add in this order) | Volume (μl) | Final Concentration |
|---|---|---|
| dA-tailed DNA w/ beads | 30 | |
| 5X Thermo Rapid Ligation Buffer | 10 | 1X |
| Y-Adaptor (2.5 μM) | 6 | 0.3 μM |
| T4 DNA ligase (5 U/ μl) | 4 | 0.4U / μL |
| Total Volume | 50 |
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