| Reagents (add in this order) | Volume (μL) | Final Concentration |
|---|---|---|
| Nuclei w/ beads | 169 | |
| 10X T4 ligase buffer w/ ATP | 20 | 1X |
| 10 mM dNTPs | 5 | 0.25 mM |
| T4 DNA Polymerase (3U/ μl) | 3 | 0.045 U / μL |
| Klenow (10U/ μl) | 3 | 0.15U / μL |
| Total Volume | 200 |
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