| Reagents (add in this order) | Volume (μL) | Final Concentration |
|---|---|---|
| Nuclei w/ beads | 135 | |
| 10X NEBuffer 2 | 20 | 1X |
| 10 mM dATP | 10 | 0.5 mM |
| 10% Triton X-100 | 20 | 1% |
| Klenow (exo-) (5U/ μl) | 15 | 0.375U / μL |
| Total Volume | 200 |
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