| Reagents (add in this order) | Volume (μL) | Final Concentration |
|---|---|---|
| Nuclei w/ beads | 80 | |
| 10X T4 ligase buffer w/ ATP | 10 | 1X |
| PNK (10 U/ μl) | 10 | 1U / μL |
| Total Volume | 100 |
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