| Reagents (add in this order) | Volume (μl) | Final Concentration |
|---|---|---|
| H2O | 794 | |
| 10X T4 ligase buffer | 100 | 1X |
| T4 DNA ligase (5 U/ μl) | 6 | 0.03U / μL |
| Total Volume | 1 mL |
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