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Levels of dimerized IRF3 in rotavirus-infected cells. Lysates prepared from infected Caco-2 cells were electrophoretically resolved under nondenaturing or denaturing conditions. Western blot analysis was used to locate monomer and dimer forms of IRF3 in the nondenaturing gel and IRF3, wtNSP1, and proliferating cell nuclear antigen (PCNA) in the denaturing gel.
