Fig. 7.

Effect of NSP1 on EGFP-IRF3 levels. Equivalent amounts of pEGFP or pEGFP-IRF3 were cotransfected with pCI, pCI-NSP1, pCI-NSP1ΔC17, or pCI-NSP1ΔC71 into 293T cells to drive the expression of either EGFP or EGFP-IRF3 alone or together with wtNSP1 or NSP1ΔC proteins. (A) Epifluorescence microscopy (×10) was used to analyze the EGFP signal (green). (B) Flow cytometry analysis was used to calculate percentage of total cells that were fluorescent. The data are represented in density plot graphics where the upper left quadrant corresponds to nonfluorescent cells and the upper right quadrant corresponds to fluorescent cells. The percentage of cells in each group is given in the corresponding quadrant.