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Reduction in IRF3 degradation by a proteasome inhibitor. Appropriate plasmids were transfected into 293T cells to drive the expression of EGFP-IRF3 alone or together with wtNSP1. The transfected cells or mock-transfected cells were then incubated with media containing 2.5 μM protease inhibitor MG132 dissolved in DMSO or DMSO alone. After 16 h, the percentage of luminescent cells in the population was determined by flow cytometry.
