Figure 2. Local Flt3L potentiates IP checkpoint modulation against bilateral TRAMP-C2.

(A) Five to eight-week-old C57BL/6 mice were challenged with 1×10⁶ TRAMP-C2 cells on both flanks, and were treated on days 14, 18, and 22 post-implantation with intraperitoneal (IP) checkpoint modulators and/or intratumoral (IT) Flt3L at either the right (R) or both right and left flank tumors (R&L). (B) Percent of mice tumor free at each flank, (C) tumor growth kinetics, and (D) overall survival over the treatment period are shown. Mice were deemed moribund when tumor volume reached 1000 mm³. Data is cumulative of n = 3 experiments with 5 mice per group. Statistical significance for survival was calculated using the Gehan-Breslow-Wilcoxon test. ns = not significant, * = P < 0.05, ** = P < 0.01, *** = P < 0.001, **** = P < 0.0001.