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TFE3 overexpression in ACHN cells. Visualization of ACHN cells in the OE group (A and B) prior to puromycin selection, and (C and D) following 48 h of puromycin selection. Magnification, ×100. (E) Western blot analysis of TFE3 expression in the three experimental groups. FLAG-tagged TFE3 was detected at 95 kDa. TFE3, transcription factor E3; OE, overexpression; NC, negative control; Con, control.
