Fig. 2.

A functional TRKA protein is up-regulated in AML1-ETO-positive cells. (a) Protein lysates from three AML1-ETO-positive cell cultures (lanes 4–6) and three control cultures (lanes 1–3) were analyzed for expression of TRKA. The CB cells and the three AML1-ETO-positive cell cultures were selected for CD34⁺ cells. The two PBPC cultures were already predominantly CD34⁺. 293T and 293T-TRKA were included as controls. (b) Cells from an actively growing AE9 culture were rested overnight without cytokines, and then treated with 50 ng/ml NGF. Cell lysates were subjected to IP with an anti-TRKA antibody, and Western blots were probed for anti-phosphotyrosine (4G10) and anti-TRKA. (c) The protein lysates were probed by Western blot for activated ERK and activated AKT, which became phosphorylated in response to NGF.