Table 1. NGF synergizes with the five-cytokine mix (KTF36) to promote expansion of AML1-ETO-positive cells.
| Total cell expansion × 1013
|
CD34+ cell expansion × 1013
|
|||||||
|---|---|---|---|---|---|---|---|---|
| KTF36 | KTF36 + NGF(20) | KTF36 + NGF(1) | Fold increase | KTF36 | KTF36 + NGF(20) | KTF36 + NGF(1) | Fold increase | |
| Week 9 AE9 | 2.3 | 17.9 | 7.8 | 1.3 | 10.3 | 8.2 | ||
| Week 9 AE11.1 | 6.5 | 22.6 | 3.5 | 1.9 | 7.2 | 3.7 | ||
| Week 10 AE13 | 2.5 | 9.3 | 3.8 | 1.1 | 3.8 | 3.6 | ||
| Week 11 AE11.1 | 6.1 | 17.6 | 2.9 | 2.2 | 4.6 | 2.1 | ||
The AML1-ETO-positive cells were CD34-selected at the indicated week of growth and expanded in liquid culture using the five-cytokine mixture KTF36 or additionally supplemented with 20 ng/ml NGF or 1 ng/ml NGF. Cells were counted weekly by Trypan blue dye exclusion and stained for expression of CD34. The numbers indicate growth over a 10-week period. Control CB and PBPC CD34+ cells were cultured under similar conditions, with no effect of NGF on total or CD34+ cell expansion (data not shown).