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. 2017 Jul 24;40(3):731–738. doi: 10.3892/ijmm.2017.3079

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Analysis of apoptosis by flow cytometry. Two NCAPG siRNAs were transiently transfected into HCC cells and the apoptosis of MHCC-LM3 and Hu7 cells was assessed by flow cytometric apoptosis assay. (A) The number of apoptosis NCAPG siRNA-transfected MHCC-LM3 and Hu7 cells was significantly higher than the negative control (NC) groups. (B) Western blot showing that the levels of Bax and cleaved caspase-3 were increased, and the levels of Bcl-2 were reduced in the NCAPG siRNA-transfected groups. NCAPG, non-SMC condensin I complex subunit G; HCC, hepatocellular carcinoma.