Figure 4.

Gene expression patterns and Web Gene Ontology Annotation Plot (WEGO) in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-exposed subventricular zone (SVZ) at different time points. (A) Identification of MPTP-induced changes in gene expression in the SVZ corresponding to the different temporal expression profiles. There were two different gene expression patterns: i) cluster A, genes with long-lasting upregulation throughout the experimental periods [pyruvate dehydrogenase kinase, isozyme 4 (Pdk4) and G protein-coupled receptor 17 (Gpr17)]; followed by ii) cluster B, genes with a significant decrease at the early stage but further overrepresented 3 weeks after MPTP [nuclear receptor subfamily 4, group A, member 3 (Nr4a3), Fos-like antigen 2 (Fosl2), early growth response 2 (Egr2), Egr4 and solute carrier family 17 (vesicular glutamate transporter), member 7 (Slc17a7)]. (B) The annotation analysis of transcriptomic changes in the SVZ was plotted and compared between two datasets of day 4 and week 3 by WEGO. Gene Ontology (GO) pathways with >3 genes were considered significant and included in the figure. In this histogram, significantly differential genes (FPKM >1, fold change >1 or <−1, q-value <0.05) were compared between the day 4 and week 3 group.