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. 2017 Jun 30;16(3):2445–2454. doi: 10.3892/mmr.2017.6894

Figure 5.

Figure 5.

Expression of cancer-related factors was altered by caffeine treatment. MGC-803 and SGC-7901 cells were obtained via enzymatic digestion following caffeine treatment for 24 h. Human gastric mucosa epithelial cells (GES-1) were used as an internal control. Relative mRNA expression levels were detected by reverse transcription-quantitative polymerase chain reaction, and the data were analysed using the 2−ΔΔCq method. (A) Relative mRNA expression levels of β-catenin, PTEN, AKT, mTOR, p53 and VEGF-A in MGC-803 and SGC-7901 cells were measured after 24 h of caffeine treatment. GES-1 cells were used the untreated normal control. (B) Relative mRNA expression levels of β-catenin, PTEN, AKT, mTOR, p53 and VEGF-A in MGC-803 and SGC-7901 cells were measured and recorded at the indicated time points after caffeine withdrawal. Data are representative of at least three independent experiments. *P<0.01 and **P<0.01 vs. control. PTEN, phosphatase and tensin homolog; mTOR, mammalian target of rapamycin; VEGF-A, vascular endothelial growth factor-A; AKT, protein kinase B.