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. Author manuscript; available in PMC: 2017 Aug 8.
Published in final edited form as: Oncogene. 2014 Aug 4;34(23):3023–3035. doi: 10.1038/onc.2014.239

Figure 3.

Figure 3

WEE1 interacts with APC/C. (a) Flag-WEE1 was immunoprecipitated and immunoblots of interacting proteins of the APC/C complex are shown. (b, c) Reciprocal immunoprecipitation of ectopically expressed Cdh1 (left) and Cdc20 (right) with WEE1 by using antibodies either against HA-tagged Cdh1 and Cdc20 (b) or flag-tagged WEE1 (c). (d, e) Co-immunoprecipitation of endogenous WEE1 with APC/C after immunoprecipitating endogenous Cdc27 in HeLa cells (d) as well as overexpressed Cdc20 and Cdh1 in 293T cells (e).