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CtsL degradation of soluble α-syn at pH 5. (A) Schematic representation of the primary amino acid sequence of α-syn (residues 1 to 140) with LC-MS mapped cleavage sites from CtsL activity after 15 min (red) and 23 h (black) incubation. (B) Observed peptide fragments derived from CtsL degradation of α-synm (15 μM) after 15 min incubation.
