Figure 3.
BMSC-EVs increased HUVEC viability, tube formation and migration in vitro. (A) Viability of HUVECs in the absence (control) or presence of two concentrations of BMSC-EVs, as determined by Cell Counting Kit-8 assay. (B) Tube formation of HUVECs in the absence (control) or presence of two doses of BMSC-EVs. Scale bar, 200 µm. (C) Quantitative analysis of the vessel-like structures formed in response to the two doses of BMSC-EVs. (D) Cell migration of HUVECs in the absence (control) or presence of two concentrations of BMSC-EVs. Scale bar, 200 µm. (E) Quantitative analysis of the recovered area after a scratch test in response to two doses of BMSC-EVs (n=9/group). *P<0.05. BMSC-EVs, bone marrow mesenchymal stem cell-derived extracellular vesicles; HUVECs, human umbilical vein endothelial cells; OD, optical density.