Table I.

Quantitative polymerase chain reaction primers and product sizes.

Amplicon	Primers	Accession no.	Genomic location	Gene symbol	Tm (°C)	Length (bp)
GAPDH(human)	F: 5′GGGAAACTGTGGCGTGAT3′	NM_001289746	Chr 12	GAPDH	60	299
	R: 5′GAGTGGGTGTCGCTGTTGA3′
hsa_circ_0000144	F: 5′GAAGGTAGGACAAATGGAAGTT3′	NM_052931	Chr 1	SLAMF6	60	136
	R: 5′GAATCTGCTTAGTTCTACCTCTC3′
hsa_circ_0023642	F: 5′ATGACAAACTGACGGAAAAGGAG3′	NM_003369	Chr 11	UVRAG	60	64
	R: 5′AACCAAGGGCAACAGCAATG3′
hsa_circ_0032821	F: 5′AGATAGAAAGGCAGGAGCAG3′	NM_152446	Chr 14	CEP128	60	146
	R: 5′TGTTCAGTCTCCAAGCAAAG3′
hsa_circ_0005529	F: 5′AGTCCCTGCGCCTCATCTTG3′	NM_018668	Chr 15	VPS33B	60	101
	R: 5′CGCCGCTCTAGCACCTTTCT3′
hsa_circ_0061274	F: 5′CAGCCTTCTCAATTTTCTTTC3′	NM_003489	Chr 21	NRIP1	60	96
	R: 5′AGTCTTCAGATTCCCTGTCCT3′
hsa_circ_0000026	F: 5′CCATCCCCTTATTCAGCACAT3′	NM_001198803	Chr 1	EIF4G3	60	132
	R: 5′TCCAAACTTCAGTTTCCTCATCA3′
hsa_circ_0040039	F: 5′CAGGATACTTGTTCAGGGTTGC3′	NM_006750	Chr 16	SNTB2	60	201
	R: 5′TTGGTGCTGTTCTGGTGTTTT3′
hsa_circ_0041732	F: 5′GCTCACATGCCCACCCATTA3′	NM_019013	Chr 17	FAM64A	60	127
	R: 5′CAGCCACTTGGTGCCACTTT3′
hsa_circ_0068610	F: 5′GACAATGCTGCTTTCCCTTTC3′	NM_003234	Chr 3	TFRC	60	154
	R: 5′CCAGTAACCGGATGCTTCACA3′
hsa_circ_0005927	F: 5′TGAATTTGGAGGTTCTATCTACCAG3′	NM_001135694	Chr 8	VDAC3	60	162
	R: 5′CCTTCAATTTCCCACTCTTCTTT3′

circ, circular RNA; F, forward; R, reverse; Chr, chromosome; Tm, melting temperature; bp, base pairs.