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. 2017 Jul 15;16(3):3095–3102. doi: 10.3892/mmr.2017.7016

Figure 4.

Figure 4.

(A) Details of the following four ATBF1 expression vector constructs: The HA-ATBF1 vector, which contains the full-length ATBF1 sequence; the ATBF1-2.7N vector, which contains the N-terminal fragment of ATBF1 (amino acids 1–892) and does not possess any NLS; the ATBF1-7.2M vector, which contains the middle region of ATBF1 (amino acids 893–3,290), and possesses three NLS; and the ATBF1-1.2C vector, which contains the C-terminal fragment of ATBF1 (amino acids 3,291–3,703) and does not possess any NLS. (B) Confocal microscopy images of HCT116 cells transfected with the four HA-tagged ATBF1 expression vectors. Transfection with the full-length ATBF1 vector (HA-ATBF1) and the vector containing the middle region of the ATBF1 protein (ATBF1-7.2M) were detected in the nucleus, whereas the N-terminal (ATBF1-2.7N) and C-terminal (ATBF1-1.2C transfection) fragment vectors were detected in the cytoplasm. ATBF1, AT motif binding factor 1; HA, haemagglutinin; NLS, nuclear localization signal.