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Culture of normal B cells with CD40L-expressing feeder cells. HT-1080 cells were modified to express CD40L, as confirmed by (A) flow cytometry. (B) HT-1080 pLVX-CD40L cells were used as feeder cells to stimulate the proliferation of normal B cells. Images were captured using an Nikon Eclipse TE2000-E microscope, magnification, ×200. CD40, cluster of differentiation 40 ligand; PE, phycoerythrin.
