Figure 2.

Lentiviral modification of normal B cells and Raji cells. (A) Three vectors (pLVX ZsGreen1, pLVTHM and pGIPZ) were used for lentiviral modification of (B) normal B cells or (C) Raji cells. The percentage of GFP-positive cells was determined using flow cytometry. Co-cultures of normal B cells and feeder cells were additionally stained with anti-CD20 antibody to distinguish B cells from feeder cells. GFP, green fluorescent protein; CMV, cytomegalovirus; EF1α, elongation factor 1 alpha; CD20, cluster of differentiation 20; PE, phycoerythrin.