Table 2.

MIC distribution patterns, ECOFFs and range of the different clusters in the whole data collection.

Class (subclass)	Antimicrobial	MIC distribution pattern	ECOFF (μg/mL)	Wild type cluster (μg/mL)	Non wild type clusters (μg/mL)
Beta lactams
(Penams)	Amoxicillin	Unknown	0.12*	≤0.03–0.12	0.5–4
	AMC	Unknown	0.25*	≤0.03–0.25	0.5–4
	Penicillin	Unknown	0.03*	≤0.03	0.06–4
(Cephalosporins)	Ceftiofur	Unimodal	NA	0.03–8	NA
	Cefquinome	Unimodal	NA	0.002–0.5	NA
Amphenicols	Florfenicol	Unimodal	NA	0.5–4	NA
Pleuromutilins	Tiamulin	Multi-modal	2	0.06 – >2	4 – >64
Tetracyclines	Tetracycline	Multi-modal	4	0.25–4	16 – >128
	Doxycycline	Multi-modal	0.5	0.06–0.5	4–64
	Erythromycin	Multi-modal	0.12	0.015–0.12	2 – >32
Macrolides	Tylosin	Multi-modal	4	0.25–4	128 – >256
	Tilmicosin	Multi-modal	32	0.5–32	>128
Lincosamides	Lincomycin	Multi-modal	0.25*	0.06–0.25	0.5 – >128
Aminocyclitol	Spectinomycin	Multi-modal	64	4–64	≥512
Fluoroquinolones	Enrofloxacin	Multi-modal	NA	NA	0.12–4 and >8
	Marbofloxacin	Multi-modal	0.015	0.015	0.25–2 and 16
Potentiated sulphonamides	TMPS	Multi-modal	0.12*	0.015–0.12	0.25 – >32

Note: Antibiotics including florfenicol, ceftiofur and cefquinome presented a unimodal distribution therefore ECOFF values were not set and NWT cluster were not considered. Wild-type cluster was not considered in the case of enrofloxacin.

AMC: Amoxicillin/Clavulanate. TMPS: Trimethoprim/Sulfamethoxazole. NA: Not applicable (ECOFF were unable to be defined in this sample set).

^*Tentative cut-off for the most susceptible cluster.