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. Author manuscript; available in PMC: 2017 Aug 8.
Published in final edited form as: Int J Cancer. 2009 Aug 15;125(4):826–836. doi: 10.1002/ijc.24399

Figure 2. Overexpression or knockdown of Cdh1 alters the Skp2-p27 cascade in breast cancer cells.

Figure 2

a) (Upper panel) Overexpression of Cdh1 resulted in the downregulation of Skp2 and the upregulation of p27 in breast cancer cells. (Lower panel) Quantification of the expression of Cdh1, Skp2 and p27. b) (Upper panel) Engineering of the Skp2 knockdown clone using shRNA in breast cancer cells. (Lower panel) Quantification of immunoblotting analysis repeated at least three times. c) Depletion of Skp2 resulted in the attenuation of p27 levels. d) Depletion of Skp2 induced upregulation of p27 while additional overexpression of Cdh1 did not alter the levels of p27 in Skp2-knockdown breast cancer cells. e) Depletion of Cdh1 increased the levels of Skp2. However, depletion of both Cdh1 and Skp2 showed no effect on the levels of p27 in MCF7 cells (Representative figures from at least three-independent analysis were shown here.)