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. Author manuscript; available in PMC: 2017 Aug 8.
Published in final edited form as: Kidney Int. 2016 Oct 10;91(2):375–386. doi: 10.1016/j.kint.2016.08.020

Figure 3. Interleukin (IL)-4/IL-13 was essential for macrophage/dendritic cell polarization in response to diphtheria toxin–mediated acute kidney injury.

Figure 3

(a) Flow cytometric analysis gating with F4/80 indicated that there were more renal macrophages (F4/80+ CD11b+ CD11c−) in DTR; IL-4/IL-13 knockout (KO) mice than in diphtheria toxin receptor (DTR) mice at 6 days after diphtheria toxin injection. *P < 0.05 versus DTR mice, n = 6. (b) Further cytometric analysis showed that CD206+ macrophages (difference between F4/80+CD11b+ CD206+ cells and F4/80+ CD11c+ CD206+ cells) accounted for a much smaller percentage of total macrophage in DTR; IL-4/IL-13 KO mice. **P < 0.01 versus DTR mice, n = 8. (c) IL-4/IL-13 KO also led to increased renal neutrophil infiltration at 6 days after DT injection. **P < 0.01 versus DTR mice, n = 6. (d) Isolated renal macrophages/dendritic cells from DTR; IL-4/IL-13 KO mice had lower mRNA levels of IL-4Rα and CD209 (a marker of M2a),=but had comparable mRNA levels of B7-H4 and CD150 (markers of M2c) compared with that in DTR mice. *P < 0.05 versus DTR mice, n = 5. MR, mannose receptor.