Skip to main content
. Author manuscript; available in PMC: 2017 Aug 8.
Published in final edited form as: Kidney Int. 2016 Oct 10;91(2):375–386. doi: 10.1016/j.kint.2016.08.020

Figure 4. Interleukin (IL)-4/IL-13 promoted macrophage/dendritic cell M2 polarization at 3 weeks after diphtheria toxin (DT)-mediated acute kidney injury.

Figure 4

Figure 4

(a) There was increased renal epithelial expression of CX3CL-1 (fractalkine), a macrophage-homing chemokine, in DT receptor (DTR); IL-4/IL-13 knockout (KO) mouse kidney at 3 weeks after DT administration. Bar = 100 μm. (b) Macrophage density was much higher in both renal cortex and medulla in DTR; IL-4/IL-13 KO mice 3 weeks after DT administration. ***P < 0.001 versus DTR mice, n = 4. Bar = 100 μm. (c) IL-4/IL-13 deletion led to decreased renal expression levels of IL-4Rα and arginase 1 (Arg1), markers of M2 phenotypic macrophages, but increased α-smooth muscle actin (SMA) levels at 3 weeks after DT injection. **P < 0.01, ***P < 0.001 versus DTR mice, n = 3. (d) Immunostaining indicated decreased renal CD206-positive macrophages in DTR; IL-4/IL-13 KO mice at 3 weeks after DT injection. Arrows: CD206-positive macrophages. ***P < 0.001 versus DTR mice, n = 4. Bar = 70 μm. MR, mannose receptor; pf, per field.