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. Author manuscript; available in PMC: 2017 Sep 20.
Published in final edited form as: Biochemistry. 2016 Sep 7;55(37):5230–5242. doi: 10.1021/acs.biochem.6b00482

Figure 3.

Figure 3

Site transfer probabilities and kinetic activity of hOGG1 in the presence of variable cosolutes. (A) The multistep search and repair pathway of DNA glycosylases involves associative and dissociative translocation along the DNA chain (see text). (B) Phosphorimages of the products derived from reaction of hOGG1 with two 90mer substrates (S20oxoG and S10oxoG) with two oxoG residues positioned 10 and 20bp apart. Solution conditions and co-solutes are indicated under each lane of the gel images. (C) The transfer probabilities between oxoG sites in S10oxoG and S20oxoG in the presence of variable cosolutes using 30 mM (red) and 150 mM salt (black) (T = 37°C). (D) Effects of salt, 20% PEG 8K, and salDNA on the observed rate of 8-oxoG excision from S20oxoG. Error is derived from 3 independent trials of each condition.