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. Author manuscript; available in PMC: 2017 Sep 20.
Published in final edited form as: Biochemistry. 2016 Sep 7;55(37):5230–5242. doi: 10.1021/acs.biochem.6b00482

Table 2.

Analysis of the salt dependences of non-specific and specific binding of hOGG1.a

Slope (N)b Kanon (M−1)c
KaN −1.0 ± 0.2 4.0 ± 0.1 × 105
−1.3 ± 0.3d 8.8 ± 0.6 × 104d
KaTATA −1.9 ± 0.4 2.6 ± 0.2 × 104
KaGC −2.0 ± 0.5 2.5 ± 0.3 × 104
KaGCGC −1.8 ± 0.4 5.4 ± 0.4 ×104
KaS −2.1 ± 0.3d 1.5 ± 0.1 × 104d
a

All experiments were conducted using WT hOGG1 unless otherwise noted and the error is derived from the linear fit.

b

The slope (N) obtained from nonlinear regression fitting to eq 3.

c

Kanon is the extrapolated value of the Ka to the condition of 1 M [K+].

d

These values were determined using K249Q hOGG1.