. Author manuscript; available in PMC: 2017 Sep 20.

Published in final edited form as: Biochemistry. 2016 Sep 7;55(37):5230–5242. doi: 10.1021/acs.biochem.6b00482

Table 3.

Electrostatic (ΔG_elect) and non-electrostatic (ΔG_non) contributions to the binding free energy (ΔG_bind) for non-specific (D^N, D^TATA, D^GC, D^GCGC) and specific (D^S) hOGG1 complexes in the presence of 150 mM K⁺.^a

	D^N	D^TATA	D^GC	D^GCGC	D^S
ΔG_bind^b (kcal mol⁻¹)	−8.6 ± 0.4	−8.2 ± 0.3	−8.4 ± 0.3	−8.5 ± 0.3	−7.9 ± 0.4^e
ΔG_bind^b (kcal mol⁻¹)	−8.0 ± 0.2^e	−8.2 ± 0.3	−8.4 ± 0.3	−8.5 ± 0.3	−7.9 ± 0.4^e
ΔG_elect^c (kcal mol⁻¹)	−1.1 ± 0.6	−2.3 ± 0.4	−2.5 ± 0.7	−2.2 ± 0.5	−2.3 ± 0.8
ΔG_elect^c (kcal mol⁻¹)	−1.4 ± 0.6^e	−2.3 ± 0.4	−2.5 ± 0.7	−2.2 ± 0.5	−2.3 ± 0.8
ΔG_non^d (kcal mol⁻¹)	−7.5 ± 0.2	−5.9 ± 0.1	−5.9 ± 0.4	−6.3 ± 0.2	−5.6 ± 0.4
ΔG_non^d (kcal mol⁻¹)	−6.6 ± 0.4^e	−5.9 ± 0.1	−5.9 ± 0.4	−6.3 ± 0.2	−5.6 ± 0.4

All values are derived from experiments using KOAc.

Calculated ΔG_bind = −RT ln K_a, using K_a at 150 mM [K⁺].

ΔG_elect = ΔG_bind - ΔG_non. ΔG_elect pertains to the condition of 150 mM [K⁺].

ΔG_non = −RT ln K_a, using the measured K_a at 1M [K⁺].

These values were determined using K249Q