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. 2016 Oct 4;5:1849454416663648. doi: 10.1177/1849454416663648

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© The Author(s) 2016

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — Specificity of EV capture and staining. (a) EVs were captured from untreated PPP either with MHC-I-MNPs (red) or with isotype control mouse immunoglobulin G (MsIgG)-MNPs (gray) and stained with anti-CD41-APC and anti-CD9-PE antibodies (left panel); EVs were captured from untreated PPP with MHC-I-MNPs and stained for isotype control antibodies mouseIgG1k-APC and mouseIgG1k-PE (right panel). (b) EVs were captured from PPP treated with ExoQuick either with MHC-I-MNPs (red) or with isotype control MsIgG-MNPs (gray) and stained with anti-CD41-APC and anti-CD9-PE (left panel); EVs were captured from PPP treated with ExoQuick with MHC-I-MNPs and stained for isotype control antibodies mouseIgG1k-APC and mouseIgG1k-PE (right panel). (c) EVs were captured from PPP treated with thrombin and ExoQuick, either with MHC-I-MNPs (red) or with isotype control MsIgG-MNPs (gray) and stained with anti-CD41-APC and anti-CD9-PE antibodies (left panel); EVs were captured from PPP treated with thrombin and ExoQuick with MHC-I-MNPs and stained for isotype control antibodies mouseIgG1k-APC and mouseIgG1k-PE (right panel). (d) EVs were captured from PPP treated with thrombin either with MHC-I-MNPs (red) or with isotype control MsIgG-MNPs (gray) and stained with anti-CD41-APC and anti-CD9-PE (left panel); EVs captured from PPP treated with thrombin with MHC-I-MNPs and stained for isotype control antibodies mouseIgG1k-APC and mouseIgG1k-PE (right panel). EV: extracellular vesicle; PPP: platelet-poor plasma; MHC-I: major histocompatibility complex class-I; MNP: magnetic iron oxide nanoparticle; CD: cluster of differentiation.