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M. tuberculosis-secreted PI3P phosphatase SapM inhibits phagosome–late endosome fusion in vitro. The standard phagosome–late endosome in vitro fusion reaction was carried as described (5), in the presence of 32 μg/ml of purified SapM (Mt SapM). (Inset) Fusion reaction in the presence of 100 nM wortmannin (Wm). Ctrl, untreated control. Error bars represent SEM. *, P < 0.05, n = 3.
