Table 1.

The MM CTC assay is repeatable across titration points. Intra-assay variability was calculated for each titration point from triplicates stained on three separate days. High consistency within the assay (low intra-assay variability, % CV<20) was demonstrated for most conditions. High % CV for n = 1 and 10 was expected due to high variability for detecting low number of cells.

Run	Expected	Mean	St dev	% CV
1	1	1	0.6	43.3
	10	9	1.5	17.6
	100	62	9.1	14.7
	1000	994	26.1	2.6
2	1	1	1.2	173.2
	10	5	0.6	10.8
	100	57	5.7	10.0
	1000	948	46.2	4.9
3	1	0	0.6	173.2
	10	7	3.5	49.5
	100	59	8.7	14.9
	1000	951	23.3	2.5