Figure 5.
S100B release in patients and healthy individuals after bacteria stimulation. S100B release, determined by ELISA assay, after 24 hours of stimulation with enteroinvasive Escherichia coli (EIEC) and/or L. casei DG® (Lactobacillus paracasei CNCM I-1572; LCDG) in colonic mucosa of (a) healthy controls, (b) patients with diverticulosis, (c) SUDD and (d) SUDD with previous episodes of AD (SUDD + AD). EIEC + LCDG: LCDG added 2.5 hours after EIEC; LCDG + EIEC: EIEC added 2.5 hours after LCDG; EIEC and LCDG: EIEC and LCDG added contemporaneously. All results are expressed as mean ± SEM of n = 10 experiments performed in triplicate. * p < 0.05 vs CTRL; ** p < 0.05 vs EIEC. ELISA: enzyme-linked immunosorbent assay; SUDD: symptomatic uncomplicated diverticular disease; AD: acute diverticulitis; CTRL: control; a.u.: arbitrary units.