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. Author manuscript; available in PMC: 2017 Aug 8.
Published in final edited form as: Gene Ther. 2014 Nov 27;22(2):209–215. doi: 10.1038/gt.2014.104

Figure 3.

Figure 3

Antigen-specific effector functions of CD19-CAR T-cells in vitro. (a) IFN-γ secretion by CD19-CAR T-cells after co-culture with 3T3/CD19 or parental 3T3 cells. (b) Cytotoxicity of CD19-CAR T-cells against Raji and K562 cells in Calcein-AM release assays. K562 cells served as CD19 targets. (c) Cytotoxic activities in CD4- and CD8-sorted T-cells. (d) Cytotoxicity against primary CD19+ diffuse large B-cell lymphoma (DLBCL) cells. Values show the mean ±s.d. of triplicate wells.