Skip to main content
. 2005 Mar 7;102(11):3966–3971. doi: 10.1073/pnas.0501063102

Fig. 2.

Fig. 2.

Effects of various STAT1 mutations on IFN-γ activation and subsequent inactivation. (A) Time course (min) of IFN-γ activation of cells expressing STAT1α or mutants assayed by EMSA. (B) Time course (hr) of activation of STAT1 and mutants assayed by EMSA. (C) Persistent tyrosine phosphorylation of mutant STAT1. U3A cells were transiently transfected (or not) with the indicated expression vectors and treated (or not) with IFN-γ for 0.5 or 6 h. Western blotting was with anti-pY STAT1 antibody or STAT1 antibody. (D) Failure to bind DNA, accomplished with the N460A mutation, does not affect the prolonged phosphorylation phenotype. Reconstituted U3A cells were treated with IFN-γ for 0.5 or 5 h. A portion of the extracts was subjected to EMSA (Left) whereas the remainder was analyzed for tyrosine phosphorylation by Western blotting with anti-pY STAT1 antibody (Right). Anti-STAT1 Western blots show the total expressed protein levels.