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. Author manuscript; available in PMC: 2017 Aug 8.
Published in final edited form as: Cell Rep. 2017 Jun 20;19(12):2586–2597. doi: 10.1016/j.celrep.2017.05.080

Figure 1. PKN1-deficiency attenuates neutrophil adhesion to endothelial cells.

Figure 1

A. Validation of the lack of PKN1 proteins in neutrophils isolated from Pkn1m/m mice by Western blot analysis. PKN2 and tubulin were detected as internal controls.

B–D. PKN1-deficiency impairs neutrophil infiltration into inflamed mouse peritonea (B), adhesion to endothelial cells under shear flow (C), and binding to ICAM1 (D). Data are presented as means ± SEM (*, p<0.05, Student’s t-Test, n>5)

E–G. Effect of PKN1-deficiency on neutrophil-endothelial cell interaction in inflamed cremaster muscle venues. Adhesion (E), transmigration of neutrophils (F), and rolling flux (G), were determined after stimulation of the cremaster muscle with TNFα (0.5 µg) for 4h. All values are means of n = 5 animals (with 5 to 7 vessels per animal) ± SEM. (*, p<0.05; **, p<0.01, Student’s t-Test).