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. 2005 Mar 17;102(13):4807–4812. doi: 10.1073/pnas.0409177102

Fig. 3.

Fig. 3.

Functional characterization of nonsynonymous KLF11 variants. (A) GAL4 reporter assay with N-terminal KLF11-GAL4DBD constructs cotransfected in CHO cells (n = 4). (Upper) A representative Western blot of coimmunoprecipitation experiments (n = 2) showing mSin3a binding of Flag-tagged KLF11 (WT and variants) or empty vector (control). (B) Luciferase assays of Flag-tagged KLF11 variants cotransfected with human insulin promoter reporter constructs in βTC3 cells (n = 3). (C) Real-time PCR quantification of insulin mRNA expression in βTC3 cells cultured in high-glucose medium (25 mM) and transfected with control, WT KLF11, or 62R-KLF11. Values are corrected for GAPDH levels (n = 4). *, significantly different (P < 0.05).