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. Author manuscript; available in PMC: 2018 Aug 3.
Published in final edited form as: Mol Cell. 2017 Jul 20;67(3):374–386.e5. doi: 10.1016/j.molcel.2017.06.023

Figure 6. RADX depletion in BRCA2-deficient cells prevents nascent strand degradation. See also Figure S5.

Figure 6

(A) U2OS cells or (B) CAPAN-1 cells transfected with siRNA, or (C) U2OS cells overexpressing RADX (OE) were labeled with IdU and CldU prior to treatment with 3mM HU for five hours. The lengths of DNA fibers were scored and plotted as histograms and dot plots. P-values were derived using Mann Whitney test. Experiments are representative of at least two repeats. Figure S5E lists the numbers of fibers analyzed. (D and E) The percentage of DR-GFP-positive U2OS cells after transfection with siRNAs and I-SceI expression vector. (Mean+/−SD, n=3 in which 25,000 cells were scored per experiment). (F) The percentage of SA-GFP-positive U2OS cells after transfection with siRNAs and I-SceI expression vector. (Mean+/−SD, n=3 in which 25,000 cells were scored per experiment). (G) The number of sister chromatid exchanges per chromosome were scored in U2OS cells transfected with siRNAs. The experiment is representative of three repeats. The number of chromosomes analyzed is indicated in parentheses.